Ep0087404a2 a process for the production of dextran. Regulations food and drug administration fda iron dextran complex is a prescription drug subject to labeling and other requirements. Extraction of dextran was carried out using ethanol. The turbidity of dextran haze was measured by reading in spectrophotometer at a wave length of 720nm. Quantitative study of the production of dextran from sucrose. Farwa sarwat 1, shah ali ul qader 2, afsheen aman 3, nuzhat ahmed 1. Historically, dextrans had been long recognized as contaminants in sugar processing and other food production. The direct production of ethanol from dextran industrial waste water was investigated by using zymomonas mobilis via batch and semicontinuous fermentation mode. Dextran for clinical and technical products is produced in most developed countries throughout the world. Production of dextransucrase, dextran and fructose from. Maximum yield of dextran was obtained when using ground date seeds. Effect of ph on dextran production is shown in fig. Reliable figures for the annual world production of. Dextran is the name given to a large class of extracellular bacterial polysaccharides composed almost exclusively of glucose units linked predominantly by 1.
Read production and structural characterisation of dextran from an indigenous strain of leuconostoc mesenteroides ba08 in whey, international journal of dairy technology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. The former strains convert sucrose into dextran with the dextransucrase. The production of dextran and fructose from carob pod extract cpe and cheese whey cw as carbon source by the bacterium leuconostoc mesenteroides was investigated. Production and characterization of dextran from leuconostoc mesenteroides nrrl b512f fermentation. Dextran can be used as a stabilizing coating to protect metal nanoparticles from oxidation and improve biocompatibility. Abstract the application of commercial dextranases to break down dextran in u. Production of high viscosity dextranthe viscosity of dextran from. Time course for dextran and dextransucrase production by leuconostoc mesenteroides cmg7 using 2% sucrose medium. Dextran dextrinase and dextran of gluconobacter oxydans. Low potassium dextran lung preservation solution reduces. Pharmacosmos sells and ships directly to clients everywhere in the world. Certain strains of gluconobacter oxydans have been known since the 1940s to produce the enzyme dextran dextrinase ddase. Dextran is manufactured under the influence of bacteria, mostly of the genus leuconostoc, which have. The residue limit for lead and arsenic in white sugar set by the codex alimentarius is 0.
Iron dextran complex canada sri 2009 and is available from. The dextran haze was produced by diluting analiquot of the treated, filtered solution, to twice the aliquot volume by the addition of ethanol. Pdf fermentative production of dextran using food industry wastes. Xanthan gum is a microbial polysaccharide of great commercial significance. Investigations on the influence of dextran during beet sugar. Production of high viscosity dextran the viscosity of dextran from leuconostoc mesenteroides nrrl b512 was found to be influenced greatly by cultural conditions. Continuous production of dextran from immobilized cells of. Abstract in the present study, the production of dextran from date extract and sucrose as carbon sources by the bacterium leuconostoc mesenteroides nrrl b512 f was investigated. Similarly, in the present work, the effect of different ph values of culture medium on dextran production was studied where maximum dextran production figure 3 by ba08 was observed at 6. Method for the production of dextran comprising the following steps. The former strains convert sucrose into dextran with the dextransucrase enzyme whereas the latter convert maltodextrins into.
A study of dextran production from maltodextrin by cell. Institute of sustainable halophytes utilization, university of karachi75270, pakistan 3. The major raw material used for the production of dextran is sucrose. Low potassium dextran solution did not alter the membrane potential. Characterization of the dextran produced by leuconostoc. Optimum conditions and composition of culture media for dextran production using sugar. A large number of lactic acid bacteria lab are known to produce dextran. A novel process for the production of clinical dextran a dissertation submitted to the graduate faculty of the louisiana state university and agricultural and mechanical college in partial fulfillment of the requirements for the degree of doctor of philosophy in the department of microbiology by doman kim b.
The global leader in pharmaceutical dextran pharmacosmos. Exposure during production may be sitelimited, because only one manufacturer of iron dextran was identified in the united states in 2009 sri 2009. Specimens of pamylase limit dextrin, soluble starch, amylopectin, native b512 dextran, and a commercial preparation of low about 80,000 molecular weight clinical dextran did not affect reaction rate or oligosaccharide production appre. Ls1and li1 isolated from sauerkraut and idli batter was selected for dextran production. Dextran is manufactured under the influence of bacteria, mostly of the genus leuconostoc, which have the ability of synthesizing dextran out of saccharose. Maximum dextransucrase production was found in 20 hrs incubation and sharp decline in production was observed in 24 hrs. The use of dextran will be limited to bakery products, i. Dextran, which is a high molecular substance built up from glucose units with cil,6glycoside linkages, is predominantly used as synthetic blood plasma. Aug 31, 1983 dextran, which is a high molecular substance built up from glucose units with cil,6glycoside linkages, is predominantly used as synthetic blood plasma.
A quantitative study of the production of dextran from sucrose by rumen strains of streptococcus bovis by r. This characteristic branching distinguishes a dextran from a dextrin, which is a straight chain glucose polymer tethered by. In 2001, the european union authorized the addition of dextran containing more than 95% of 16 linkages and a molar mass higher than 2x10 6 gmol as food ingredient in bakery products. Dextran was again precipitated with cold ethanol as described above, and the precipitate was dried in a desiccator. Effect of temperature on production of oligosaccharides. In agreement with the literature the experimental data show that the dextran has a negligible effect on the sucrose solubility. Using the spectrophotometric method, as a new method, the influence of dextran on the sucrose solubility and metastable zone width has been studied. Production of high viscosity dextranthe viscosity of dextran from leuconostoc mesenteroides nrrl b512 was found to be influenced greatly by cultural conditions. In the literature, there has been very little work done on dextran nanofiber production by electrospinning. The results also show that this impurity decreases the sucrose metastable zone width.
The method was calibrated against a commercially available dextran. The molecular weight and concentration of dextran sulfate. Apr 25, 2016 to investigate the effect of dextran sulfate ds, a widely used anti. Small family 5 to 20 members, it was placed in the endangered category according to iucn in 1960. Freshly isolated and old stock strains of streptococcus bovis originating. Nov 01, 2016 read production and structural characterisation of dextran from an indigenous strain of leuconostoc mesenteroides ba08 in whey, international journal of dairy technology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Pdf dextran is a dglucose polymer which is produced by leuconostoc mesenteroides grown in a sucroserich media. The formation of dextran with high molecular weight was measured by hplcri. May 22, 2019 dextran 40 is a hypertonic colloidal solution. Dextran has biocompatibility and biodegradability characteristics, which are very important properties in biomedical applications such as drug delivery 11, 12 and scaffolds. The optimum sucrose concentration for the leuconostoc spp. Factors affecting production and clarification of dextran. We deliver pharmaceutical quality dextran of the highest standards, including those of the european pharmacopoeia ep, the united states pharmacopoeia usp, and the japanese pharmacopoeia jp.
Production of reactive oxygen species as measured by chemiluminescence was signi. Production of dextran from free and immobilized cells of leuconostoc mesenteroides kibge ha1 using 10% sucrose in acrylamide. It was probably the first biopolymer produced on an industrial scale by fermentation when production started around 1948. The present report describes a effects of some factors on dextran production, b factors affecting enzymatic degradation of dextran through extended incubation, and c dextran opalescence and methods of. Pdf production and characterization of dextran from leuconostoc. Pdf direct ethanol production from dextran industrial waste.
To investigate the effect of dextran sulfate ds, a widely used anti. The best results were obtained when 20% wv molasses and 15% wv wheat bran were used. A novel process for the production of clinical dextran. In the last steps of the synthesis water is used as solvent. A quantitative study of the production of dextran from sucrose by. The influence of secondary carbon sources maltose, lactose and galactose on. The optimum conditions for dextran and fructose production. Gillian eggleston and adrian monge2 srrcusdaars, new orleans, louisiana, u. A quantitative study of the production of dextran from.
Dextran has been used in immobilization in biosensors. Fermentative production of dextran using leuconostoc spp. To enhance the yield of dextran, effects of various parameters such as sucrose concentration, ph, temperature, incubation and inoculum percentage were analyzed. Different isolated strains of leuconostoc mesenteroides produce dextran compared with that of l. They found that higher sucrose levels induced better enzyme production but the cultures contained so much dextran that the. The formation of dextran in wine was shown by pasteur to be due to the activity of microbes. Different concentrations of date seeds were applied and the highest dextran production was achieved at 6 gflask. Production and structural characterisation of dextran from an.
Dextran chains are of varying lengths from 3 to 2000 kilodaltons. These experiments were performed to gain an insight into the time span needed to examine dextran production from g. In comparison to blue dextran mw2000 da, dextran molecular weight was reduced mw pdf available january 2010. Found in the wild, but for the commercial production to receive seed, rs 0 per piglet to.
Dextrans can be formed by many microorganisms and are not welldefined substances with specific properties. A short article about dextran and its effects on the sugar industry rafrogblogus. Pdf solidstate fermentation for the production of dextran. However, when these conditions were controlled, dextrans of high or of low viscosity were obtained as desired. Pdf fermentative production of dextran using food industry.
Sucrose dextran dextran is a complex, branched glucan polysaccharide made of many glucose molecules composed of chains of varying lengths. Leuconostoc mesenteroides nrrl b512 was found to be influenced greatly by cultural. Ls1 and li1 was found to be 15% and 25% respectively. Centre for molecular genetics, university of karachi, pakistan 2. Investigations of the influence of dextran on sugar cane. Pdf production of dextran and fructose from carob pod. Sigmaaldrich offers a number of dextran sulfate sodium salt products. Miers from the starch and dextrose division, northern regional research laboratory, peoria, illinoist received for publication, june 21, 1948.
515 179 45 558 972 1566 1444 837 837 1375 1078 1239 1177 335 111 152 1549 1351 170 1334 516 1386 67 11 878 948 547 1323 107 1443 919 1204 609 843 39 1084 1037